Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated FCGR2A-383H

Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated

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Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated

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Specification Gene Information Related Products

Cat.No. :	FCGR2A-383H
Product Overview :	Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1). Predicted N-terminus: Ala 36. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source :	HEK293
Species :	Human
Tag :	His & Avi
Form :	Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity :	Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore T200) (QC tested). Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated on SA Chip can bind Rituximab with an affinity constant of 0.74 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Molecular Mass :	24.0 kDa
Protein length :	Ala 36 - Ile 218
Endotoxin :	Less than 1.0 EU per μg by the LAL method.
Purity :	>90% as determined by SDS-PAGE.
Notes :	Please avoid repeated freeze-thaw cycles.
Stability :	No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution.
Storage :	For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name :	FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
Official Symbol :	FCGR2A
Synonyms :	FCGR2A; CD32; CD32A; CDw32; IGFR2; fcRII-a; fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032;
Gene ID :	2212
mRNA Refseq :	NM_001136219
Protein Refseq :	NP_001129691
MIM :	146790
UniProt ID :	P12318