Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated
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|Product Overview :||Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1). Predicted N-terminus: Ala 36. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Tag :||His & Avi|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity :||Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore T200) (QC tested).
Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated on SA Chip can bind Rituximab with an affinity constant of 0.74 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
|Molecular Mass :||24.0 kDa|
|Protein length :||Ala 36 - Ile 218|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Notes :||Please avoid repeated freeze-thaw cycles.|
|Stability :||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name :||FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]|
|Official Symbol :||FCGR2A|
|Synonyms :||FCGR2A; CD32; CD32A; CDw32; IGFR2; fcRII-a; fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032;|
|Gene ID :||2212|
|mRNA Refseq :||NM_001136219|
|Protein Refseq :||NP_001129691|
|UniProt ID :||P12318|
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