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Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated
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Cat.No. : | FCGR2A-383H |
Product Overview : | Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1). Predicted N-terminus: Ala 36. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.0 kDa. The protein migrates as 30-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Source : | HEK293 |
Species : | Human |
Tag : | His & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 0.66 μM as determined in a SPR assay (Biacore T200) (QC tested). Recombinant Human FCGR2A Protein (H167), His & Avi-tagged, Biotinylated on SA Chip can bind Rituximab with an affinity constant of 0.74 μM as determined in a SPR assay (Biacore T200) (Routinely tested). |
Molecular Mass : | 24.0 kDa |
Protein length : | Ala 36 - Ile 218 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Figure : |
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Gene Name : | FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ] |
Official Symbol : | FCGR2A |
Synonyms : | FCGR2A; CD32; CD32A; CDw32; IGFR2; fcRII-a; fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032; |
Gene ID : | 2212 |
mRNA Refseq : | NM_001136219 |
Protein Refseq : | NP_001129691 |
MIM : | 146790 |
UniProt ID : | P12318 |
◆ Recombinant Protein
FCGR2A-384H | Recombinant Human FCGR2A Protein (R167) (Ala 36 - Ile 218), His & Avi-tagged, Biotinylated | +Inquiry |
FCGR2A-932R | Recombinant Rat FCGR2A Protein (Met1-Leu216), His-tagged | +Inquiry |
FCGR2A-3992H | Recombinant Human FCGR2A Protein, GST-tagged | +Inquiry |
FCGR2A-3990H | Recombinant Human FCGR2A Protein | +Inquiry |
FCGR2A-7236H | Recombinant Human FCGR2A, His-tagged | +Inquiry |
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◆ Lysates
FCGR2A-001HCL | Recombinant Human FCGR2A cell lysate | +Inquiry |
FCGR2A-1926CCL | Recombinant Cynomolgus FCGR2A cell lysate | +Inquiry |
FCGR2A-1996HCL | Recombinant Human FCGR2A cell lysate | +Inquiry |
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