Recombinant Human FCGR2A(H167) protein, His-Avi-tagged

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Recombinant Human FCGR2A(H167) protein, His-Avi-tagged

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Cat.No. : FCGR2A-333H
Product Overview : Recombinant Human Fc gamma RIIA / CD32a (H167) Protein is expressed from mammalian with a His tag and Avi tag at the C-terminal. It contains Ala 36 - Ile 218 (H167).
Description : The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Source : HEK293
Species : Human
Tag : His-Avi
Form : Supplied as 0. 22um filtered solution in 20mMPB,150mMNaCl, pH 7. 5.
Molecular Mass : The protein has a predicted MW of 23. 2 KDa. Due to glycosylation, the protein migrates to 30-40 KDa based on Bis-Tris PAGE result.
Protein length : It contains Ala 36 - Ile 218 (H167).
Endotoxin : Less than 0. 01 EU per ug by the LAL method.
Purity : > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Storage : The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Tris-Bis PAGE :

HPLC data :

Gene Name : FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
Official Symbol : FCGR2A
Synonyms : CD32 antigen; CD32a; CD32MGC23887; CDw32fc-gamma-RIIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RIIA; FCG2; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a
Gene ID : 2212
mRNA Refseq : NM_001136219
Protein Refseq : NP_001129691
MIM : 146790
UniProt ID : P12318

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