Cat. No. : |
FGF5-172H |
Description : |
Fibroblast Growth Factor-5 (FGF-5) , is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. |
Source : |
E.Coli. |
Supplied : |
Sterile filtered and the the protein was lyophilized from 2X PBS pH7.2( no additives). |
Purity : |
>95% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-5. |
Reconstitution : |
The lyophilized FGF-5 should be reconstituted in 1X PBS pH7.2 to a concentration of 1ug/ul (1mg/ml). Allow to set at least 30 minutes at 4°C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions or stored at 4°Cfor up to 1 week or stored at - 20°Cfor future use. For most in-vitro applications, human FGF-5 exerts its biological activity in the concentration range of 0.1 to 20ng/ml. |
Note : |
If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration >50ug/ml. |
Reactivity : |
The biological activity was determined by the does- dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 <1ng/ml, corresponding to a specific activity of >1 X 106 (1 million) units/mg. |
Amino Acid Sequence : |
MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAASL GSQGSGLEQS SFQWSPSGR RTGSLYCRVG IGFHLQIYPDG KVNGSHEANM LSVLEIFAVSQ GIVGIRGVFSN KFLAMSKKGK LHASAKFTDD CKFRERFQEN SYNTYASAIHR TEKTGREWYV ALNKRGKAKR GCSPRVKPQH ISTHFLPRFK QSEQPE LSFTV TVPEK KNPPSP IKSK IPLSAPRKNT NSVKYRLKFRFG |
Storage : |
The lyophilized powder is stable at room temperature but it is best stored desiccated below 0°C. Reconstituted human FGF-5 should be stored in working aliquots at -20℃. |