Recombinant Human FTH1

• Cat.No.: FTH1-528H
• Product Overview: Recombinant FTH1 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 21kDa (183aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Ferritin, heavy polypeptide 1 (FTH1) is a ubiquitous protein which plays a major role in iron sequestration, detoxification and storage. FTH1 is found in all living species. FTH1 encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains.
• Amino Acid Sequence: MTTASTSQVR QNYHQDSEAA INRQINLELY ASYVYLSMSY YFDRDDVALK NFAKYFLHQS HEEREHAEKL MKLQNQRGGR IFLQDIKKPD CDDWESGLNA MECALHLEKN VNQSLLELHK LATDKNDPHL CDFIETHYLN EQVKAIKELG DHVTNLRKMG APESGLAEYL FDKHTLGDSD NES.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Porphyrin and chlorophyll metabolism; Membrane Trafficking
• Publications:
  Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections (2022)

Gene Information

• Gene Name: FTH1 ferritin, heavy polypeptide 1 [ Homo sapiens ]
• Synonyms: ferritin, heavy polypeptide 1; FHC; FTH; PLIF; FTHL6; PIG15; MGC104426; FTH1; apoferritin; placenta immunoregulatory factor; proliferation-inducing protein 15; EC 1.16.3.1; apoferritin; Ferritin H subunit
• Gene ID: 2495
• mRNA Refseq: NM_002032
• Protein Refseq: NP_002023
• MIM: 134770
• UniProt ID: P02794
• Chromosome Location: 11q13
• Function: ferric iron binding; ferroxidase activity; oxidoreductase activity; protein binding

Citation

Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections

Journal: Nature Communications    PubMed ID: 35091550    Data: 2022/1/28

Authors: Brady T. Hickerson, Tracy R. Daniels-Wells, Brian B. Gowen

Article Snippet:Erythroblasts (5 × 10 5 ) were incubated with 5 μg human diferric Tf conjugated to Alexa Fluor? 546 (Thermo Fisher Scientific, Cat. No. T23364) or 3 μg of recombinant H-Ft conjugated to R-phycoerythrin (H-Ft-PE; Creative BioMart, Shirley, NY, USA, Cat. No. FTH1-528H-PE) for 60 min on ice.. Competing antibodies were added to some samples and included 10 μg of either anti-dansyl IgG1/κ isotype control antibody , ch128.1/IgG1, or the murine anti-hTfR1 IgG2a/κ antibody clone M-A712 (BD Biosciences, Cat. No. 555534) that is known to block H-Ft binding .Competing antibodies were added to some samples and included 10 μg of either anti-dansyl IgG1/κ isotype control antibody , ch128.1/IgG1, or the murine anti-hTfR1 IgG2a/κ antibody clone M-A712 (BD Biosciences, Cat. No. 555534) that is known to block H-Ft binding .