Recombinant Human GAD2 protein, His-tagged

• Cat.No.: GAD2-3178H
• Product Overview: Recombinant Human GAD2 protein(200-550 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 200-550 aa
• AA Sequence: ANTNMFTYEIAPVFVLLEYVTLKKM REIIGWPGGSGDGIFSPGGAISNMY AMMIARFKMFPEVKEKGMAALPRLI AFTSEHSHFSLKKGAAALGIGTDSV ILIKCDERGKMIPSDLERRILEAKQ KGFVPFLVSATAGTTVYGAFDPLLA VADICKKYKIWMHVDAAWGGGLLMS RKHKWKLSGVERANSVTWNPHKMMG VPLQCSALLVREEGLMQNCNQMHAS YLFQQDKHYDLSYDTGDKALQCGRH VDVFKLWLMWRAKGTTGFEAHVDKC LELAEYLYNIIKNREGYEMVFDGKP QHTNVCFWYIPPSLRTLEDNEERMS RLSKVAPVIKARMMEYGTTMVSYQP L
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: GAD2 glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) [ Homo sapiens ]
• Official Symbol: GAD2
• Synonyms: GAD2; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2; GAD65; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; MGC161605; MGC161607
• Gene ID: 2572
• mRNA Refseq: NM_000818
• Protein Refseq: NP_000809
• MIM: 138275
• UniProt ID: Q05329