Recombinant Human GCGR Protein, His-tagged
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|Product Overview :||Recombinant Human GCGR Protein (125 AA) with His tag was expressed in E.coli.|
|Description :||The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).|
|Molecular Mass :||14.9 kDa (calculated)|
|AA Sequence :||MQVMDFLFEK WKLYGDQCHH NLSLLPPPTE LVCNRTFDKY SCWPDTPANT TANISCPWYL PWHHKVQHRF VFKRCGPDGQ WVRGPRGQPW RDASQCQMDG EEIEVQKEVA KMYSSFQLEH HHHHH|
|Endotoxin :||< 1.0 EU/μg|
|Purity :||≥ 95%|
|Applications :||WB, ELISA|
|Quality Control Test :||BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
|Notes :||This product is intended for research use only.|
|Stability :||Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week.|
|Storage :||Store the lyophilized protein at –80 centigrade.|
|Storage Buffer :||Filtered (0.4 μm) and lyophilized from solution in acetonitrile/0,1%TFA + 1% (w/v) trehalose|
|Reconstitution :||Add 200 µL of deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.|
|Shipping :||At ambient temperature.|
|Gene Name :||GCGR glucagon receptor [ Homo sapiens (human) ]|
|Official Symbol :||GCGR|
|Synonyms :||GCGR; glucagon receptor; GGR; GL-R; glucagon receptor|
|Gene ID :||2642|
|mRNA Refseq :||NM_000160|
|Protein Refseq :||NP_000151|
|UniProt ID :||P47871|
◆ Recombinant Protein
|GCGR-518M||Recombinant Mouse GCGR Protein (27-143 aa), GST-tagged||+Inquiry|
|GCGR-2338M||Recombinant Mouse GCGR Protein (27-143 aa), His-tagged||+Inquiry|
|GCGR-2488R||Recombinant Rat GCGR Protein||+Inquiry|
|GCGR-5989HCL||Recombinant Human GCGR 293 Cell Lysate||+Inquiry|
◆ Assay kits
|Kit-1252||cAMP GCGR CHO-K1 GPCR Assay Kit||+Inquiry|
|Kit-1250||GCGR CHO-K1 β-Arrestin GPCR Assay Kit||+Inquiry|