Recombinant Human GMCSF/IL-3 Fusion Protein

• Cat.No.: IL3-88H
• Product Overview: Recombinant Human GMCSF/IL3 fusion protein produced in E.Coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kD.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The analog of GMCSF/IL3 is PIXY321. Results indicate that PIXY321 can stimulate multilineage hematopoiesis in vivo and enhance neutrophil and platelet recovery following chemotherapy and bone marrow transplantation (BMT). These results suggest that PIXY321 elicits the biological effects of both its component cytokines and represents a novel means of delivering two independent but interactive cytokines in combination. PIXY321 is purified by proprietary chromatographic techniques.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (c) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized after extensive dialysis against 10 mM PBS, pH= 7.4±0.1 and 0.1% BSA.
• Solubility: It is recommended to reconstitute the lyophilized PIXY321 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.2 ng/ml, corresponding to a Specific Activity of 5 x10⁶IU/mg.
• Stability: Lyophilized PIXY321although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution PIXY321should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.

Gene Information

• Synonyms: PIXY321; GMCSF/ IL3

Citation

Epitope and Fc-Mediated Cross-linking, but Not High Affinity, Are Critical for Antitumor Activity of CD137 Agonist Antibody with Reduced Liver Toxicity

Journal: Molecular Cancer Therapeutics    Data: 2020/1/23

Authors: Sun K. Ho, Zhenghai Xu, Yoshiko Akamatsu

Article Snippet:Rat monoclonal mAbs were generated according to standard hybridoma technology.Rat monoclonal mAbs were generated according to standard hybridoma technology.. Recombinant mouse and human CD137-human Fc fusion (Creative BioMart) and mouse CD137-His proteins were generated in-house and used as immunogens.. Hybridoma were screened for antigen specific binding as well as for the ability to activate NFB by a reporter assay using HEK-293 cells transduced with pLenti- NFB-Luciferase vector and a plasmid expressing human CD137.Hybridoma were screened for antigen specific binding as well as for the ability to activate NFB by a reporter assay using HEK-293 cells transduced with pLenti- NFB-Luciferase vector and a plasmid expressing human CD137.