Recombinant Human GPC3 Protein (Gln 25 - His 559), Fc-tagged, FITC-Labeled


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Recombinant Human GPC3 Protein (Gln 25 - His 559), Fc-tagged, FITC-Labeled

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Cat.No. : GPC3-338H
Product Overview : Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Gln 25 - His 559 (Accession # P51654). It is the FITC labeled form of Human Glypican 3, Fc Tag. Predicted N-terminus: Gln 25. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.4 kDa. The protein migrates as 43 kDa, 58 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 3-5.
Source : HEK293
Species : Human
Tag : Fc
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Anti-Human GPC3 MAb at 10 μg/mL (100 μL/well) can bind Recombinant Human GPC3 Protein, Fc-tagged, FITC-Labeled with a linear range of 0.078-1.25 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
Molecular Mass : 87.4 kDa
Protein length : Gln 25 - His 559
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : GPC3 glypican 3 [ Homo sapiens ]
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; SDYS; glypican-3; DGSX; OCI 5; SGB; SGBS; SGBS1; MXR7; OCI-5; GTR2-2;
Gene ID : 2719
mRNA Refseq : NM_001164617
Protein Refseq : NP_001158089
MIM : 300037
UniProt ID : P51654

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