Recombinant Human GPC3 Protein, C-6His-Avi-tagged, Biotinylated, Endotoxin-Free

• Cat.No.: GPC3-186H
• Product Overview: Recombinant Human GPC3 Protein(P51654-1) (Met1-His559) was expressed in HEK293 with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 1-559 aa
• Conjugation/Label: Biotin
• Form: Supplied as sterile PBS, pH 7.4.
• Molecular Mass: The recombinant Human GPC3 consists of 561 amino acids and predicts a molecular mass of 64.05 kDa. The band migrates approximately 70-75 kDa in the SDS-PAGE under reducing conditions.
• Endotoxin: < 0.5 EU per mg protein.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_001164617
• Protein Refseq: NP_001158089
• MIM: 300037
• UniProt ID: P51654