Recombinant Human GUCY2C protein, His-Avi-tagged

• Cat.No.: GUCY2C-092H
• Product Overview: Recombinant Human GUCY2C protein(P25092-1)(Ser24-Gln430), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ser24-Gln430
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GUCY2C, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: GUCY2C guanylate cyclase 2C (heat stable enterotoxin receptor) [ Homo sapiens ]
• Official Symbol: GUCY2C
• Synonyms: GUCY2C; guanylate cyclase 2C (heat stable enterotoxin receptor); GUC2C; heat-stable enterotoxin receptor; STAR; GC-C; hSTAR; STA receptor; guanylyl cyclase C; intestinal guanylate cyclase
• Gene ID: 2984
• mRNA Refseq: NM_004963
• Protein Refseq: NP_004954
• MIM: 601330
• UniProt ID: P25092