Recombinant Human GUCY2C protein, His-tagged
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|Product Overview :||Recombinant Human GUCY2C protein (Ser24-Gln430) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293).|
|Description :||GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.|
|Predicted N Terminal :||Ser24|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 47.9 kDa. The protein migrates as 55-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Ser24-Gln430|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.|
|SDS-PAGE of GUCY2C-670H :||
Human GUCY2C, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of GUCY2C-670H :||
Immobilized Human GUCY2C, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
|Bioactivity-FACS of GUCY2C-670H :||
2e5 of anti-GUCY2C CAR-293 cells were stained with 100 µL of 1 µg/mL Human GUCY2C, His Tag and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).
|Gene Name :||GUCY2C|
|Official Symbol :||GUCY2C|
|Synonyms :||GUCY2C,GUC2C,STAR,STA receptor,hSTAR,GC-C|
|Gene ID :||2984|
|mRNA Refseq :||NM_004963.4|
|Protein Refseq :||NP_004954.2|
|UniProt ID :||P25092|
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