Recombinant Human H3.3 Histone Protein, K27M

• Cat.No.: H3.3-327H
• Product Overview: Full length Recombinant Human H3.3 Histone Protein is produced in E. coli and in which Lys27 was mutated to Met

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. The histone variant H3.3 replaces conventional H3.1 in a wide range of nucleosomes in active genes. H3.3 constitutes the predominant form of histone H3 in non-dividing cells. The K27M mutation was identified in several human pediatric glioblastoma tumors.
• Form: 20 mM sodium phosphate pH 7.0, 0.3 M NaCl, 1mM EDTA, 0.5 mM PMSF and 1 mM DTT.
• Purity: Purified using FPLC, >98% purity as determined by SDS-PAGE.
• Notes: This product is for research use only. Not for use in diagnostic or therapeutic procedures.
• Stability: Guaranteed stable for 2 years from date of receipt when stored properly.
• Storage: Store at -80 centigrade.
• Concentration: 1 µg/µl
• Full Length: Full L.