| Description : |
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His/GST |
| Form : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Molecular Mass : |
85kDa as determined by SDS-PAGE reducing conditions. |
| Protein length : |
Met1~Pro488 |
| AA Sequence : |
MAYSQGGGKKKVCYYYDGDIGNYYY GQGHPMKPHRIRMTHNLLLNYGLYR KMEIYRPHKATAEEMTKYHSDEYIK FLRSIRPDNMSEYSKQMQRFNVGED CPVFDGLFEFCQLSTGGSVAGAVKL NRQQTDMAVNWAGGLHHAKKSEASG FCYVNDIVLAILELLKYHQRVLYID IDIHHGDGVEEAFYTTDRVMTVSFH KYGEYFPGTGDLRDIGAGKGKYYAV NFPMRDGIDDESYGQIFKPIISKVM EMYQPSAVVLQCGADSLSGDRLGCF NLTVKGHAKCVEVVKTFNLPLLMLG GGGYTIRNVARCWTYETAVALDCEI PNELPYNDYFEYFGPDFKLHISPSN MTNQNTPEYMEKIKQRLFENLRMLP HAPGVQMQAIPEDAVHEDSGDEDGE DPDKRISIRASDKRIACDEEFSDSE DEGEGGRRNVADHKKGAKKARIEED KKETEDKKTDVKEEDKSKDNSGEKT DTKGTKSEQLSNP |
| Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
| Purity : |
> 90% |
| Applications : |
Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.) |
| Notes : |
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures. |
| Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
| Storage : |
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
