| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
His |
| Protein Length : |
657-1,123 |
| Description : |
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. |
| Molecular Mass : |
51 kDa |
| Endotoxin : |
<1.0 EU/μg, determined by the LAL endotoxin assay |
| Purity : |
≥90% estimated by SDS-PAGE |
| Unit Definition : |
One unit is the amount of enzyme required to release 1 pmol of acetate per minute at 37 centigrade in 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/mL BSA, and 20 μM fluorogenic HDAC class 2a substrate. |
| Stability : |
≥ 6 months |
| Storage : |
Store it at -80 centigrade |
| Storage Buffer : |
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol |
