Recombinant Human HDAC5 Protein, His-tagged

• Cat.No.: HDAC5-48H
• Product Overview: Recombinant human HDAC5(657-1,123) protein with a C-terminal His-tag was expressed in baculovirus expression vector system.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Insect cells
• Species: Human
• Tag: His
• Molecular Mass: 51 kDa
• Protein length: 657-1,123
• Endotoxin: <1.0 EU/μg, determined by the LAL endotoxin assay
• Purity: ≥90% estimated by SDS-PAGE
• Unit Definition: One unit is the amount of enzyme required to release 1 pmol of acetate per minute at 37 centigrade in 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/mL BSA, and 20 μM fluorogenic HDAC class 2a substrate.
• Stability: ≥ 6 months
• Storage: Store it at -80 centigrade
• Storage Buffer: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol

Gene Information

• Gene Name: HDAC5 histone deacetylase 5 [ Homo sapiens (human) ]
• Official Symbol: HDAC5
• Synonyms: HDAC5; histone deacetylase 5; HD5; NY-CO-9; histone deacetylase 5; antigen NY-CO-9
• Gene ID: 10014
• mRNA Refseq: NM_001015053
• Protein Refseq: NP_001015053
• MIM: 605315
• UniProt ID: Q9UQL6