Recombinant Human HDAC5 Protein, His-tagged HDAC5-48H

Recombinant Human HDAC5 Protein, His-tagged

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Recombinant Human HDAC5 Protein, His-tagged

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Cat.No. : HDAC5-48H
Product Overview : Recombinant human HDAC5(657-1,123) protein with a C-terminal His-tag was expressed in baculovirus expression vector system.
Description : Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Source : Insect cells
Species : Human
Tag : His
Molecular Mass : 51 kDa
Protein length : 657-1,123
Endotoxin : <1.0 EU/μg, determined by the LAL endotoxin assay
Purity : ≥90% estimated by SDS-PAGE
Unit Definition : One unit is the amount of enzyme required to release 1 pmol of acetate per minute at 37 centigrade in 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/mL BSA, and 20 μM fluorogenic HDAC class 2a substrate.
Stability : ≥ 6 months
Storage : Store it at -80 centigrade
Storage Buffer : 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol
SDS-PAGE Analysis of HDAC5 :

Lane 1: MW Markers; Lane 2: HDAC5 (3 μg). Representative gel image shown; actual purity may vary between each batch.

HDAC5 Deacetylase Activity :

Gene Name : HDAC5 histone deacetylase 5 [ Homo sapiens (human) ]
Official Symbol : HDAC5
Synonyms : HDAC5; histone deacetylase 5; HD5; NY-CO-9; histone deacetylase 5; antigen NY-CO-9
Gene ID : 10014
mRNA Refseq : NM_001015053
Protein Refseq : NP_001015053
MIM : 605315
UniProt ID : Q9UQL6

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