Recombinant Human Heat Shock Protein 90kDa Beta (Grp94), Member 1

• Cat.No.: HSP90B1-07H
• Product Overview: Recombinant Human heat shock protein 90β produced in E. coli has a molecular mass of approximately 85 KDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: An approximately 90 kDa heat shock protein constitutively expressed in the cytoplasm. It exists as a dimer and has been observed to bind other cellular proteins such as p60v-src, steroid receptors, heme-regulated protein kinase, actin, and tubulin. Bind to histones with high affinity and induces chromatin condensation.
• Purity: >95% as determined by SDS-PAGE.
• Specific Activity: The biological activity ofHSP90B1 is measured by its ability to support survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Activity can also be measured by its ability to bind recombinant human GFRα1/Fc.
• Storage Buffer: Liquid. In PBS Buffer. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: HSP90B1 heat shock protein 90kDa beta (Grp94), member 1 [ Homo sapiens ]
• Synonyms: ECGP; GP96; GRP94; TRA1;94 kDa glucose-regulated protein; Endoplasmin precursor; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96); endothelial cell (HBMEC) glycoprotein; glucose regulated protein; 94 kDa; gp96 homolog; heat shock protein 90kDa beta (Grp94), member 1; tumor rejection antigen (gp96) 1
• Gene ID: 7184
• mRNA Refseq: NM_003299
• Protein Refseq: NP_003290
• MIM: 191175
• Uniprot ID: O95433
• Chromosome Location: 12q24.2-q24.3
• Pathway: Pathways in cancer; Prostate cancer
• Function: ATP binding, RNA binding, calcium ion binding, low-density lipoprotein receptor binding, nucleotide binding, unfold protein binding, virion binding

Citation

Low-concentration HCP1 inhibits apoptosis in vascular endothelial cells.

Journal: Biochemical and biophysical research communications    PubMed ID: 30770100    Data: 2019/12/10

Authors: Qun Wei, Jun Zhang, ZhaoMin Lin

Article Snippet:HCP1 was synthesized as described previously [13] and dissolved in DMSO (10mM, Sigma-Aldrich, St. Louis, Missouri, USA) as a stock solution.HCP1 was synthesized as described previously [13] and dissolved in DMSO (10mM, Sigma-Aldrich, St. Louis, Missouri, USA) as a stock solution.. Recombinant Grp94 (HSP90B1-132H) was purchased from Creative BioMart (New York, USA). . Mito-tracker deep red (M22426) was obtained from Invitrogen (Carlsbad, CA, USA).Mito-tracker deep red (M22426) was obtained from Invitrogen (Carlsbad, CA, USA).