Recombinant Human Heat Shock Protein 90kDa Beta (Grp94), Member 1

• Cat.No.: HSP90B1-338H
• Product Overview: Recombinant human GRP94 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (819 aa, 94.4 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 22-803 a.a.
• Description: GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 0.1 M NaCl, 10% glycerol.
• Molecular Weight: 94.4 kDa (819 aa).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMDDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV GTDEEEETAK ESTAEKDEL
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: HSP90B1
• Synonyms: HSP90B1; heat shock protein 90kDa beta (Grp94), member 1; HSP90B1 heat shock protein 90kDa beta (Grp94), member 1; ECGP; GP96; TRA1; GRP94; 94 kDa glucose-regulated protein; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96); endothelial cell (HBMEC) glycoprotein; glucose regulated protein, 94 kDa; gp96 homolog; heat shock protein 90kDa beta, member 1; tumor rejection antigen (gp96) 1.
• Gene ID: 7184
• mRNA Refseq: NM_003299.1
• Protein Refseq: NP_003290.1
• MIM: 191175
• UniProt ID: P14625
• Chromosome Location: 12q24.2-q24.3
• Function: ATP binding; RNA binding; calcium ion binding; low-density lipoprotein receptor binding; nucleotide binding; unfolded protein binding; virion binding.