Recombinant Human HIP1 protein, His & T7-tagged

• Cat.No.: HIP1-1738H
• Product Overview: Recombinant Human HIP1 aa. (Leu817~Glu1037 (Accession # O00291)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Leu817~Glu1037 (Accession # O00291)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 5

Gene Information

• Gene Name: HIP1 huntingtin interacting protein 1 [ Homo sapiens (human) ]
• Official Symbol: HIP1
• Synonyms: HIP1; huntingtin interacting protein 1; SHON; HIP-I; ILWEQ; SHONbeta; SHONgamma; huntingtin-interacting protein 1; huntingtin-interacting protein I
• Gene ID: 3092
• mRNA Refseq: NM_001243198.2
• Protein Refseq: NP_001230127.1
• UniProt ID: O00291