| Cat.No. : |
HRAS-85H |
| Product Overview : |
Recombinant Human HRAS Protein is produced by bacterial expression system. This protein is fused with a His tag at the amino-terminus. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
| Source : |
Bacterial |
| Species : |
Human |
| Tag : |
His |
| Form : |
2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
| Bio-activity : |
The biological activity of His-Ras can be determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to create a conformational change in the His-Ras protein that mimics GEF (Guanine nucleotide Exchange Factor) activity. |
| Molecular Mass : |
28 kDa |
| Purity : |
>80% pure |
| Applications : |
Study of Ras exchange activity with different GEFs;
Identification of Ras exchange factors (GEFs);
Positive control for GEF studies;
Biochemical characterization of Ras protein interactions;
Western blot standard. |
| Stability : |
The protein is stable for six months if stored at -70 centigrade. The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year. |
| Storage : |
Upon arrival store at 4 centigrade (desiccated). |
| Reconstitution : |
Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/ml with the addition of 20 µl of Milli-Q water. When reconstituted, the protein will be in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose and 0.1% (w/v) dextran. In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70 centigrade. |
