Active Recombinant Human HRAS Protein, His-tagged

• Cat.No.: HRAS-85H
• Product Overview: Recombinant Human HRAS Protein is produced by bacterial expression system. This protein is fused with a His tag at the amino-terminus. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.

Specification

• Species: Human
• Source: Bacteria
• Tag: His
• Form: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.
• Bio-activity: The biological activity of His-Ras can be determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to create a conformational change in the His-Ras protein that mimics GEF (Guanine nucleotide Exchange Factor) activity.
• Molecular Mass: 28 kDa
• Purity: >80% pure
• Applications: Study of Ras exchange activity with different GEFs;
  Identification of Ras exchange factors (GEFs);
  Positive control for GEF studies;
  Biochemical characterization of Ras protein interactions;
  Western blot standard.
• Stability: The protein is stable for six months if stored at -70 centigrade. The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year.
• Storage: Upon arrival store at 4 centigrade (desiccated).
• Reconstitution: Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/ml with the addition of 20 µl of Milli-Q water. When reconstituted, the protein will be in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose and 0.1% (w/v) dextran. In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70 centigrade.

Gene Information

• Gene Name: HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ]
• Official Symbol: HRAS
• Synonyms: HRAS; HRAS1; GTPase HRas; p21ras; H-Ras-1; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• Gene ID: 3265
• mRNA Refseq: NM_001130442
• Protein Refseq: NP_001123914
• MIM: 190020
• UniProt ID: P01112