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Recombinant Human HRAS Protein, His-tagged
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Cat.No. : | HRAS-85H |
Product Overview : | Recombinant Human HRAS Protein is produced by bacterial expression system. This protein is fused with a His tag at the amino-terminus. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
Source : | Bacterial |
Species : | Human |
Tag : | His |
Form : | 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
Bio-activity : | The biological activity of His-Ras can be determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to create a conformational change in the His-Ras protein that mimics GEF (Guanine nucleotide Exchange Factor) activity. |
Molecular Mass : | 28 kDa |
Purity : | >80% pure |
Applications : | Study of Ras exchange activity with different GEFs; Identification of Ras exchange factors (GEFs); Positive control for GEF studies; Biochemical characterization of Ras protein interactions; Western blot standard. |
Stability : | The protein is stable for six months if stored at -70 centigrade. The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year. |
Storage : | Upon arrival store at 4 centigrade (desiccated). |
Reconstitution : | Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/ml with the addition of 20 µl of Milli-Q water. When reconstituted, the protein will be in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose and 0.1% (w/v) dextran. In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70 centigrade. |
Gene Name : | HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ] |
Official Symbol : | HRAS |
Synonyms : | HRAS; HRAS1; GTPase HRas; p21ras; H-Ras-1; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; |
Gene ID : | 3265 |
mRNA Refseq : | NM_001130442 |
Protein Refseq : | NP_001123914 |
MIM : | 190020 |
UniProt ID : | P01112 |
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◆ Lysates
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