Recombinant Human HRAS Protein, His-tagged


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Recombinant Human HRAS Protein, His-tagged

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Cat.No. : HRAS-85H
Product Overview : Recombinant Human HRAS Protein is produced by bacterial expression system. This protein is fused with a His tag at the amino-terminus. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.
Source : Bacterial
Species : Human
Tag : His
Form : 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.
Bio-activity : The biological activity of His-Ras can be determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to create a conformational change in the His-Ras protein that mimics GEF (Guanine nucleotide Exchange Factor) activity.
Molecular Mass : 28 kDa
Purity : >80% pure
Applications : Study of Ras exchange activity with different GEFs;
Identification of Ras exchange factors (GEFs);
Positive control for GEF studies;
Biochemical characterization of Ras protein interactions;
Western blot standard.
Stability : The protein is stable for six months if stored at -70 centigrade. The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year.
Storage : Upon arrival store at 4 centigrade (desiccated).
Reconstitution : Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/ml with the addition of 20 µl of Milli-Q water. When reconstituted, the protein will be in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose and 0.1% (w/v) dextran. In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70 centigrade.
Gene Name : HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ]
Official Symbol : HRAS
Synonyms : HRAS; HRAS1; GTPase HRas; p21ras; H-Ras-1; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1;
Gene ID : 3265
mRNA Refseq : NM_001130442
Protein Refseq : NP_001123914
MIM : 190020
UniProt ID : P01112

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