Active Recombinant Human HRAS Protein, His-tagged
Cat.No. : | HRAS-85H |
Product Overview : | Recombinant Human HRAS Protein is produced by bacterial expression system. This protein is fused with a His tag at the amino-terminus. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
- Specification
- Gene Information
- Related Products
Source : | Bacterial |
Species : | Human |
Tag : | His |
Form : | 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. |
Bio-activity : | The biological activity of His-Ras can be determined from its ability to catalyze the exchange of GDP for GTP. EDTA is used to create a conformational change in the His-Ras protein that mimics GEF (Guanine nucleotide Exchange Factor) activity. |
Molecular Mass : | 28 kDa |
Purity : | >80% pure |
Applications : | Study of Ras exchange activity with different GEFs; Identification of Ras exchange factors (GEFs); Positive control for GEF studies; Biochemical characterization of Ras protein interactions; Western blot standard. |
Stability : | The protein is stable for six months if stored at -70 centigrade. The protein must not be exposed to repeated freeze-thaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year. |
Storage : | Upon arrival store at 4 centigrade (desiccated). |
Reconstitution : | Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/ml with the addition of 20 µl of Milli-Q water. When reconstituted, the protein will be in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% (w/v) sucrose and 0.1% (w/v) dextran. In order to maintain high biological activity of the protein it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment sized" amounts, snap frozen in liquid nitrogen and stored a -70 centigrade. |
Gene Name : | HRAS v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ] |
Official Symbol : | HRAS |
Synonyms : | HRAS; HRAS1; GTPase HRas; p21ras; H-Ras-1; CTLO; HAMSV; K-RAS; N-RAS; RASH1; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1; |
Gene ID : | 3265 |
mRNA Refseq : | NM_001130442 |
Protein Refseq : | NP_001123914 |
MIM : | 190020 |
UniProt ID : | P01112 |
Products Types
◆ Recombinant Protein | ||
HRAS-5023H | Recombinant Human HRAS Protein, GST-tagged | +Inquiry |
HRAS-2135H | Recombinant Human HRAS Protein | +Inquiry |
HRAS-1284R | Recombinant Rat HRAS Protein (2-186 aa), His-tagged | +Inquiry |
HRAS-2524H | Recombinant Human HRAS protein(Met1-Cys186), His-tagged | +Inquiry |
HRAS-2564R | Recombinant Rat HRAS Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
HRAS-556HCL | Recombinant Human HRAS cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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