Recombinant Human HSD3B1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HSD3B1-4934H
• Product Overview: HSD3B1 MS Standard C13 and N15-labeled recombinant protein (NP_000853) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 42.3 kDa
• AA Sequence: MTGWSCLVTGAGGFLGQRIIRLLVK EKELKEIRVLDKAFGPELREEFSKL QNKTKLTVLEGDILDEPFLKRACQD VSVIIHTACIIDVFGVTHRESIMNV NVKGTQLLLEACVQASVPVFIYTSS IEVAGPNSYKEIIQNGHEEEPLENT WPAPYPHSKKLAEKAVLAANGWNLK NGGTLYTCALRPMYIYGEGSRFLSA SINEALNNNGILSSVGKFSTVNPVY VGNVAWAHILALRALQDPKKAPSIR GQFYYISDDTPHQSYDNLNYTLSKE FGLRLDSRWSFPLSLMYWIGFLLEI VSFLLRPIYTYRPPFNRHIVTLSNS VFTFSYKKAQRDLAYKPLYSWEEAK QKTVEWVGSLVDRHKENLKSKTQTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HSD3B1 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 [ Homo sapiens (human) ]
• Official Symbol: HSD3B1
• Synonyms: HSD3B1; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; HSD3B, HSDB3; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; SDR11E1; short chain dehydrogenase/reductase family 11E; member 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5--> 4-isomerase type 1; 3-beta-HSD I; progesterone reductase; steroid Delta-isomerase; trophoblast antigen FDO161G; delta-5-3-ketosteroid isomerase; 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; short chain dehydrogenase/reductase family 11E, member 1; 4-isomerase type I; hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1; I; HSD3B; HSDB3; HSDB3A; 3BETAHSD
• Gene ID: 3283
• mRNA Refseq: NM_000862
• Protein Refseq: NP_000853
• MIM: 109715
• UniProt ID: P14060