Recombinant Human HSP90AA1 protein, His-SUMO-tagged

• Cat.No.: HSP90AA1-01H
• Product Overview: Recombinant Human HSP90AA1 protein, fused to N-terminal His tag and SUMO tag, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Liquid or Lyophilized powder
• Molecular Mass: 41.2kDa
• Protein length: 9-232aa
• AA Sequence: DQPMEEEEVETFAFQAEIAQLMSLI INTFYSNKEIFLRELISNSSDALDK IRYESLTDPSKLDSGKELHINLIPN KQDRTLTIVDTGIGMTKADLINNLG TIAKSGTKAFMEALQAGADISMIGQ FGVGFYSAYLVAEKVTVITKHNDDE QYAWESSAGGSFTVRTDTGEPMGRG TKVILHLKEDQTEYLEERRIKEIVK KHSQFIGYPITLFVEKERDKEVSD
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: Store at -20 centigrade/-80 centigrade upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Expiry: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
  Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Storage Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 centigrade/-80 centigrade. Our default final concentration of glycerol is 50%.

Gene Information

• Gene Name: HSP90AA1 heat shock protein 90 alpha family class A member 1 [ Homo sapiens (human) ]
• Official Symbol: HSP90AA1
• Synonyms: Heat shock 86 kDa; Heat shock protein 90kDa alpha cytosolic class A member 1; Heat shock protein 90kDa alpha cytosolic class B member 1; Heat shock protein HSP 90 alpha; Heat shock protein HSP 90 beta; Heat shock protein HSP 90-alpha; HS90A_HUMAN; HSP 84; HSP 86; Hsp 90; HSP86; HSP90A; HSP90AA1; HSP90AB1; HSP90B; HSPC1; HSPC2; HSPCAL1; HSPCAL4; Renal carcinoma antigen NY-REN-38.
• Gene ID: 3320
• mRNA Refseq: NM_001017963
• Protein Refseq: NP_001017963
• MIM: 140571
• UniProt ID: P07900