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Active Recombinant Human ICOS protein, His-tagged

Cat.No. : ICOS-378H
Product Overview : Recombinant Human ICOS protein(Glu 21 - Phe 141), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human B7-H2, Fc Tag at 1 μg/mL (100 μL/well) can bind Human ICOS (C136S, C137S), His Tag with a linear range of 0.02-0.313 μg/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 15.7 kDa. The protein migrates as 26-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength : Glu21-Phe141
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : ICOS inducible T-cell co-stimulator [ Homo sapiens ]
Official Symbol : ICOS
Synonyms : ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850;
Gene ID : 29851
mRNA Refseq : NM_012092
Protein Refseq : NP_036224
MIM : 604558
UniProt ID : Q9Y6W8

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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