|Product Overview :||Recombinant Human IFNA2 fused with His tag at the C-terminus was produced in Nicotiana Sp.Plant.|
|Description :||IFN-alpha is produced by macrophages and has antiviral activities. Interferon stimulates the production of two enzymes: protein kinase and an oligoadenylate synthetase.|
|Source :||Nicotiana Sp.Plant|
|Form :||Lyophilized, containing 50% SDS.|
|Bio-activity :||The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1 × 10^8 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.|
|Molecular Mass :||Interferon Alpha Human 2a Recombinant produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19 kDa.|
|AA sequence :||The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.|
|Purity :||Greater than 99.0% as determined by both:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
|Storage :||Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution IFN-alpha 2a should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
|Reconstitution :||It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.|
|Gene Name :||IFNA2 interferon, alpha 2 [ Homo sapiens ]|
|Official Symbol :||IFNA2|
|Synonyms :||IFNA2; interferon, alpha 2; interferon alpha-2; alpha 2a interferon; IFN alphaA; IFNA; interferon alpha 2b; interferon alpha A; leIF A; IFN-alpha-2; alpha-2a interferon; INFA2; IFNA2B; IFN-alphaA; MGC125764; MGC125765;|
|Gene ID :||3440|
|mRNA Refseq :||NM_000605|
|Protein Refseq :||NP_000596|
|UniProt ID :||P01563|