Recombinant Human IgE protein, His&Avi-tagged, Biotinylated

• Cat.No.: IgE-1556HB
• Product Overview: Biotinylated Human IgE Protein(P01854-1)(Cys209-Lys428) is expressed from HEK293 with His and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 209-428 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human IgE, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 32-38 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin