| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
25-91 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Insulin-like Growth Factor 2 (IGF2) is a mitogenic peptide hormone critical for fetal growth and development, predominantly synthesized by the liver and placenta. It mediates signaling through the IGF1 receptor (IGF1R) and insulin receptor isoform A (IR-A) to govern cell proliferation, differentiation, and metabolic processes. Dysregulated IGF2 expression is associated with oncogenesis and overgrowth disorders such as Beckwith-Wiedemann syndrome. IGF2 exhibits high-affinity binding to Insulin-like Growth Factor Binding Protein 5 (IGFBP5), which dynamically modulates IGF2 bioactivity by either enhancing receptor engagement or restricting ligand-receptor interaction, contingent on the cellular microenvironment.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IGF2 and recombinant rat IGFBP5 .Briefly, biotin-linked IGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IGFBP5-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IGF2 and IGFBP5 was shown, the EC50 for this effect is 0.46ug/mL. |
| Molecular Mass : |
11.2kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
