Recombinant Human IGHG1 Protein

Cat.No.: IGHG1-245H
Product Overview: Recombinant human IgG1 Fc region (AAC82527.1) (Glu 99-Lys 330) (one aa mutation,103 Cys/Ser) was expressed.
Source: HEK293
Species: Human
Predicted N Terminal: Glu 99
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its ability to bind human CD16a-His in a functional ELISA.2. Measured by its ability to bind human CD16a-AVI-His in a functional ELISA.3.Measured by its ability to bind human CD16a-His in a functional ELISA.
Molecular Mass: The recombinant human IgG1 Fc consists of 232 amino acids and has a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of rhFc is approximately 32 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) [ Homo sapiens ]
Official Symbol: IGHG1
Synonyms: IGHG1
Gene ID: 3500
mRNA Refseq:
Protein Refseq:
UniProt ID:
Chromosome Location: 14q32.33
Pathway: Classical antibody-mediated complement activation, organism-specific biosystem; Complement cascade, organism-specific biosystem; Fcgamma receptor (FCGR) dependent phagocytosis, organism-specific biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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