Active Recombinant Human IGHG1 Protein

• Cat.No.: IGHG1-245H
• Product Overview: Recombinant human IgG1 Fc region (AAC82527.1) (Glu 99-Lys 330) (one aa mutation,103 Cys/Ser) was expressed.

Specification

• Source: HEK293
• Species: Human
• Predicted N Terminal: Glu 99
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind human CD16a-His in a functional ELISA.2. Measured by its ability to bind human CD16a-AVI-His in a functional ELISA.3.Measured by its ability to bind human CD16a-His in a functional ELISA.
• Molecular Mass: The recombinant human IgG1 Fc consists of 232 amino acids and has a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of rhFc is approximately 32 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IGHG1 immunoglobulin heavy constant gamma 1 (G1m marker) [ Homo sapiens ]
• Official Symbol: IGHG1
• Synonyms: IGHG1
• Gene ID: 3500
• Chromosome Location: 14q32.33
• Pathway: Classical antibody-mediated complement activation, organism-specific biosystem; Complement cascade, organism-specific biosystem; Fcgamma receptor (FCGR) dependent phagocytosis, organism-specific biosystem