Recombinant Human IGHG3 protein

• Cat.No.: IGHG3-176H
• Product Overview: Recombinant Human IGHG3 (Glu99-Lys377) protein carried no tag and was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Protein Length: Glu99-Lys377
• Description: Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc/IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3).
• Predicted N Terminal: Glu99
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: Human IgG3-Fc/IGHG3 Region has a calculated MW of 31.2 kDa. The reducing (R) protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IGHG3
• Official Symbol: IGHG3
• Synonyms: IgG3
• Gene ID: 3502
• MIM: 147120
• UniProt ID: P01860