Recombinant Human IGHG3 Protein (SPR verified)
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| Cat.No. : | IGHG3-928H |
| Product Overview : | Recombinant Human IGHG3 protein (SPR verified)(Glu99-Lys377) was expressed in human 293 cells (HEK293). |
| Description : | Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3). |
| Source : | HEK293 |
| Species : | Human |
| Predicted N Terminal : | Glu99 |
| Form : | Tris with Glycine, Arginine and NaCl, pH7.5 |
| Molecular Mass : | The protein has a calculated MW of 31.2 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : | Glu99-Lys377 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >95% as determined by SDS-PAGE. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| SDS-PAGE of IGHG3-928H : |
Human IgG3 Fc, Tag Free (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. |
| Bioactivity-SPR of IGHG3-928H : |
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind Human IgG3 Fc, Tag Free (SPR verified) with an affinity constant of 0.683 μM as determined in SPR assay (Biacore 8K) (QC tested). |
| Gene Name : | IGHG3 |
| Official Symbol : | IGHG3 |
| Synonyms : | Ig gamma-3 chain C region,IgG,IgG3 |
| Gene ID : | 3502 |
| MIM : | 147120 |
| UniProt ID : | P01860 |
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◆ Native Protein
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