Recombinant Human IL12 Protein
|Product Overview :||Recombinant Human IL12(Arg23-Ser219&Ile23-Ser328) was produced in HEK293.|
- Gene Information
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|Description :||IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.|
|Form :||Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.|
|Protein length :||Arg23-Ser219&Ile23-Ser328|
|AA sequence :||RNLPVATPDPGMFPCLHHSQNLLRA VSNMLQKARQTLEFYPCTSEEIDHE DITKDKTSTVEACLP LELTKNESCLNSRETSFITNGSCLA SRKTSFMMALCLSSIYEDLKMYQVE FKTMNAKLLMDPKRQ IFLDQNMLAVIDELMQALNFNSETV PQKSSLEEPDFYKTKIKLCILLHAF RIRAVTIDRVMSYLN AS&IWELKKDVYVVELDWYPDAPGEMVV LTCDTPEEDGITWTLDQSSEVLGSG KTLTIQVKEFGD AGQYTCHKGGEVLSHSLLLLHKKED GIWSTDILKDQKEPKNKTFLRCEAK NYSGRFTCWWLTTIS TDLTFSVKSSRGSSDPQGVTCGAAT LSAERVRGDNKEYEYSVECQEDSAC PAAEESLPIEVMVDA VHKLKYENYTSSFFIRDIIKPDPPK NLQLKPLKNSRQVEVSWEYPDTWST PHSYFSLTFCVQVQG KSKREKKDRVFTDKTSATVICRKNA SISVRAQDRYYSSSWSEWASVPCS|
|Endotoxin :||Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.|
|Purity :||Greater than 95% as determined by reducing SDS-PAGE.|
|Storage :||Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
|Reconstitution :||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Shipping :||The product is shipped at ambient temperature.|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Fluorescence microscopy tracks IL12 internalization and trafficking.
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
Reporter gene assays analyze IL12's promoter activity and response elements.
Super-resolution imaging details IL12's cellular distribution and dynamics.
3D cell culture models reveal IL12's effects on tissue microenvironments.
Co-localization studies and co-IP assays identify IL12's binding partners.
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Transgenic models elucidate IL12's roles in immune response regulation.
Customer Reviews (3)Write a review
Effective in Th1 cell differentiation. -
High specificity in ELISA. -
Stable under storage. -
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