Recombinant Human IL12 Protein

• Cat.No.: IL12-158H
• Product Overview: Recombinant Human IL12(Arg23-Ser219&Ile23-Ser328) was produced in HEK293.

Specification

• Description: IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.
• Source: HEK293
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4.
• Protein length: Arg23-Ser219&Ile23-Ser328
• AA sequence: RNLPVATPDPGMFPCLHHSQNLLRA VSNMLQKARQTLEFYPCTSEEIDHE DITKDKTSTVEACLP LELTKNESCLNSRETSFITNGSCLA SRKTSFMMALCLSSIYEDLKMYQVE FKTMNAKLLMDPKRQ IFLDQNMLAVIDELMQALNFNSETV PQKSSLEEPDFYKTKIKLCILLHAF RIRAVTIDRVMSYLN AS&IWELKKDVYVVELDWYPDAPGEMVV LTCDTPEEDGITWTLDQSSEVLGSG KTLTIQVKEFGD AGQYTCHKGGEVLSHSLLLLHKKED GIWSTDILKDQKEPKNKTFLRCEAK NYSGRFTCWWLTTIS TDLTFSVKSSRGSSDPQGVTCGAAT LSAERVRGDNKEYEYSVECQEDSAC PAAEESLPIEVMVDA VHKLKYENYTSSFFIRDIIKPDPPK NLQLKPLKNSRQVEVSWEYPDTWST PHSYFSLTFCVQVQG KSKREKKDRVFTDKTSATVICRKNA SISVRAQDRYYSSSWSEWASVPCS
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100 μg/ml.
  Dissolve the lyophilized protein in ddH2O.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.
• Tag: Non

Reviews

01/07/2022

Effective in Th1 cell differentiation.

04/09/2020

High specificity in ELISA.

08/02/2018

Stable under storage.

Q&As

How does fluorescence microscopy track IL12 internalization and intracellular trafficking?

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines?

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles?

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements?

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide?

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments?

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context?

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry?

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks?

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation?

Transgenic models elucidate IL12's roles in immune response regulation.