| Cat.No. : |
IL12RB2-9483H
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Recombinant Human IL-12 R beta 2 protein (Lys24-Asn622) with Human IgG1 (Pro100-Lys330) and a 6-His tag at C-terminus was expressed in mouse myeloma cell line. |
| Description : |
The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Fc/His |
| Form : |
Lyophilized |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑12 is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Recombinant Human IL‑12 R beta 2 Fc that produces 50% of the optimal binding response is 0.015-0.09 µg/mL. |
| Molecular Mass : |
95 kDa |
| N-terminal Sequence Analysis : |
Lys24 |
| Endotoxin : |
<0.10 EU per 1 µg of the protein by the LAL method. |
| Purity : |
> 95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Storage Buffer : |
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose. |
| Reconstitution : |
Reconstitute at 250 µg/mL in PBS. |
| Shipping : |
The product is shipped at ambient temperature. |
| Binding Activity : |
When Recombinant Human IL-12 is immobilized at 0.5 µg/mL, Recombinant Human IL-12 R beta 2 Fc Chimera binds with an ED50 of 0.015-0.09 µg/mL.
|
| SDS-PAGE : |
1 µg/lane of Recombinant Human IL‑12 R beta 2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing bands at 112-133 kDa and 220-260 kDa, respectively.
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.