Recombinant Human IL13 protein, hFc-tagged

• Cat.No.: IL13-147H
• Product Overview: Recombinant Human IL13 protein(AAK53823)(Gly21-Asn132), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Gly21-Asn132
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8 ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL13 interleukin 13 [ Homo sapiens ]
• Official Symbol: IL13
• Synonyms: IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13
• Gene ID: 3596
• mRNA Refseq: NM_002188
• Protein Refseq: NP_002179
• MIM: 147683
• UniProt ID: P35225

Reviews

11/29/2021

Effective in Th2 cell differentiation.

04/22/2020

Good for cytokine assays.

01/26/2019

High yield in expression.

Q&As

How does flow cytometry help quantify IL13 receptor expression on different cell populations?

Flow cytometry quantifies IL13 receptor expression on cells.

What are the benefits of using transgenic animal models to study IL13's in vivo functions?

Transgenic models illuminate IL13's in vivo functions.

Could you explain the role of ELISA in quantifying IL13 secretion levels in cell cultures?

ELISA quantifies IL13 secretion in cell cultures accurately.

How can siRNA knockdown experiments reveal IL13's effects on specific cellular processes?

siRNA knockdown uncovers IL13's specific cellular effects.

How does co-immunoprecipitation identify interacting partners in IL13-mediated signaling complexes?

Co-immunoprecipitation identifies IL13 signaling partners.

What techniques can differentiate IL13-mediated signaling pathways from IL4 pathways?

Differential techniques distinguish IL13 and IL4 signaling pathways.

Could you elaborate on the use of reporter gene assays to assess IL13-driven transcriptional activity?

Reporter gene assays gauge IL13-driven transcription.

Can you describe the applications of microarray analysis in profiling IL13-induced gene expression changes?

Microarray analysis profiles IL13-induced gene expression.

What experimental methods determine the impact of IL13 on tissue remodeling processes?

Experimental methods reveal IL13's impact on tissue remodeling.

How does site-directed mutagenesis help identify critical residues in IL13 receptor binding?

Site-directed mutagenesis identifies key residues in IL13 receptor binding.