Active Recombinant Human IL17A protein, His-tagged
Cat.No. : | IL17A-2293H |
Product Overview : | Active Recombinant Human IL17A protein(Q16552)(20~155 aa), fused with N-terminal His tag, was expressed in E.coli. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 20-155 aa |
Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | IL17 (interleukin 17) is a member of IL17 cytokine family, which is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. It is reported that IL17 is a ligand for IL17RA, indicating its interaction with IL17RA. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17 with recombinant human IL17RA. Briefly, IL17 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17 with IL17RA was shown and this effect was in a dose dependent manner. |
Molecular Mass : | 19.5kDa |
Purity : | > 97% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | IL17A interleukin 17A [ Homo sapiens ] |
Official Symbol | IL17A |
Synonyms | IL17A; interleukin 17A; CTLA8, IL17, interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8); interleukin-17A; cytotoxic T lymphocyte associated protein 8; IL 17; IL 17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); IL17; CTLA8; IL-17; IL-17A; |
Gene ID | 3605 |
mRNA Refseq | NM_002190 |
Protein Refseq | NP_002181 |
MIM | 603149 |
UniProt ID | Q16552 |
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◆ Native Proteins | ||
IL17A-048H | Active Glycosylated Recombinant Human IL17A Homodimer | +Inquiry |
◆ Cell & Tissue Lysates | ||
IL17A-001HCL | Recombinant Human IL17A cell lysate | +Inquiry |
Not For Human Consumption!
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