Recombinant Human IL17RD cell lysate

• Cat.No.: IL17RD-2919HCL
• Product Overview: Human IL17RD derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: IL17RD interleukin 17 receptor D [ Homo sapiens ]
• Official Symbol: IL17RD
• Synonyms: IL17RD; interleukin 17 receptor D; interleukin-17 receptor D; FLJ35755; IL 17RD; IL17RLM; SEF; hSef; IL17Rhom; sef homolog; IL-17 receptor D; interleukin-17 receptor-like protein; IL-17RD; MGC133309; DKFZp434N1928
• Gene ID: 54756
• mRNA Refseq: NM_017563
• Protein Refseq: NP_060033
• MIM: 606807
• UniProt ID: Q8NFM7
• Chromosome Location: 3p21.1
• Pathway: FGF signaling pathway, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by FGFR, organism-specific biosystem
• Function: receptor activity

Reviews

12/22/2020

Potent in anti-inflammatory assays.

04/25/2019

High bioactivity.

04/13/2019

Good for receptor studies.

Q&As

What experimental strategies are employed to understand the expression patterns and regulation of IL17RD across different tissues and cell types?

RT-qPCR, single-cell RNA sequencing, and immunohistochemistry reveal IL17RD expression patterns and regulatory mechanisms.

What experimental methods are used to investigate the involvement of IL17RD in the crosstalk between different immune signaling pathways?

Co-stimulation assays, pathway-specific inhibitors, and gene expression profiling elucidate IL17RD's cross-pathway interactions.

What methodologies are employed to assess the impact of IL17RD on immune cell differentiation, activation, and cytokine production?

Flow cytometry, functional assays, and gene expression analysis investigate IL17RD's impact on immune cell functions.

Could you explain how genetic manipulation, such as knockout or knockdown, of IL17RD in animal models helps decipher its physiological functions?

Knockout mice, CRISPR-based models, and tissue-specific ablation provide insights into IL17RD's functions in vivo.

How are molecular and structural biology approaches utilized to uncover the binding partners or ligands that interact with IL17RD?

Yeast two-hybrid screens, co-crystallization, and surface plasmon resonance uncover IL17RD's interacting partners or ligands.

How do researchers study the potential therapeutic applications of targeting IL17RD in immune-related disorders?

Preclinical studies, therapeutic antibodies, and disease models assess IL17RD as a potential target for immune-related conditions.

How are insights from experimental studies on IL17RD translated into clinical research to evaluate its relevance in human diseases?

Clinical trials, biomarker validation, and patient stratification evaluate IL17RD's clinical relevance and potential therapies.

How do researchers experimentally distinguish IL17RD's specific contributions within the broader IL17 receptor family?

Functional assays, co-immunoprecipitation, and knockout models distinguish IL17RD's specific roles within the IL17 receptor family.

What cellular and functional assays are used to elucidate the downstream signaling events triggered by IL17RD activation and their implications for immune responses?

Reporter gene assays, phosphoprotein analysis, and cytokine profiling unveil IL17RD-mediated signaling cascades and immune outcomes.

Can you elaborate on the techniques used to investigate IL17RD's potential role as a co-receptor or modulator of IL17 signaling pathways?

Biophysical analyses, mutation studies, and chimeric receptor assays explore IL17RD's role in modulating IL17 signaling.