Recombinant Mouse Interleukin 17 receptor D, His-tagged

• Cat.No.: Il17rd-5650M
• Product Overview: RecombinantMouse Il17rd protein was expressed in Murine myeloma cellline. Gly28-Arg299,with a C-terminal 6-His tag.

Specification

• Cat. No.: Il17rd-5650M
• Description: Il17rd isa type I transmembrane protein that is found in both the cytoplasm and plasmamembrane. The gene for this protein belongs to a synexpression group originallyidentified in zebrafish and SEF is expressed along with FGF-3, -8, sprouty-2(SPRY2) and SPRY4. Due to the presence of an alternate start site, there isone transcript that potentially gives rise to two isoforms. The first is a full-lengthlong form and the second an N-terminally truncated form.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Gly28
• MolecularWeight: 31.6 kDa
• Activity: Bioassaydata are not available.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 54-60kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS containing at least 0.1% human or bovine serum albumin
• Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il17rd interleukin 17 receptorD [ Mus musculus ]
• Official Symbol: Il17rd
• Synonyms: Il17rd; interleukin17 receptor D; Sef; Sef-S; AI428510; 2810004A10Rik; interleukin-17receptor D; mSef; IL-17RD; sef homolog; IL-17 receptor D; interleukin-17receptor-like protein
• Gene ID: 171463
• mRNA Refseq: NM_134437
• Protein Refseq: NP_602319
• UniProt ID: Q3KQI3
• Chromosome Location: 14
• Function: receptor activity;zinc ion binding

Reviews

12/22/2020

Potent in anti-inflammatory assays.

04/25/2019

High bioactivity.

04/13/2019

Good for receptor studies.

Q&As

What experimental strategies are employed to understand the expression patterns and regulation of IL17RD across different tissues and cell types?

RT-qPCR, single-cell RNA sequencing, and immunohistochemistry reveal IL17RD expression patterns and regulatory mechanisms.

What experimental methods are used to investigate the involvement of IL17RD in the crosstalk between different immune signaling pathways?

Co-stimulation assays, pathway-specific inhibitors, and gene expression profiling elucidate IL17RD's cross-pathway interactions.

What methodologies are employed to assess the impact of IL17RD on immune cell differentiation, activation, and cytokine production?

Flow cytometry, functional assays, and gene expression analysis investigate IL17RD's impact on immune cell functions.

Could you explain how genetic manipulation, such as knockout or knockdown, of IL17RD in animal models helps decipher its physiological functions?

Knockout mice, CRISPR-based models, and tissue-specific ablation provide insights into IL17RD's functions in vivo.

How are molecular and structural biology approaches utilized to uncover the binding partners or ligands that interact with IL17RD?

Yeast two-hybrid screens, co-crystallization, and surface plasmon resonance uncover IL17RD's interacting partners or ligands.

How do researchers study the potential therapeutic applications of targeting IL17RD in immune-related disorders?

Preclinical studies, therapeutic antibodies, and disease models assess IL17RD as a potential target for immune-related conditions.

How are insights from experimental studies on IL17RD translated into clinical research to evaluate its relevance in human diseases?

Clinical trials, biomarker validation, and patient stratification evaluate IL17RD's clinical relevance and potential therapies.

How do researchers experimentally distinguish IL17RD's specific contributions within the broader IL17 receptor family?

Functional assays, co-immunoprecipitation, and knockout models distinguish IL17RD's specific roles within the IL17 receptor family.

What cellular and functional assays are used to elucidate the downstream signaling events triggered by IL17RD activation and their implications for immune responses?

Reporter gene assays, phosphoprotein analysis, and cytokine profiling unveil IL17RD-mediated signaling cascades and immune outcomes.

Can you elaborate on the techniques used to investigate IL17RD's potential role as a co-receptor or modulator of IL17 signaling pathways?

Biophysical analyses, mutation studies, and chimeric receptor assays explore IL17RD's role in modulating IL17 signaling.