Recombinant Human IL18 protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL18-501H
• Product Overview: Biotinylated Recombinant Human IL18 protein(Q14116) (Tyr37-Asp193) was expressed in E.coli with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi&His
• Protein Length: 37-193 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant Human IL-18 comprises 186 amino acids and has a predicted molecular mass of 21.37 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320
• Gene ID: 3606
• mRNA Refseq: NM_001243211
• Protein Refseq: NP_001230140
• MIM: 600953
• UniProt ID: Q14116

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.