Active Recombinant Human IL18 protein, His-tagged
Cat.No. : | IL18-2296H |
Product Overview : | Active Recombinant Human IL18 protein(Q14116)(37~193 aa), fused with N-terminal His tag, was expressed in E.coli. |
- Specification
- Gene Information
- Related Products
- Download
Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 37-193 aa |
Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | Interleukin-18 (IL18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. IL18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). Besides, Caspase 1 (CASP1) has been identified as an interactor of IL18, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL18 and recombinant human CASP1. Briefly, IL18 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL18 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL18 and CASP1 was shown, and this effect was in a dose dependent manner. |
Molecular Mass : | 19.5kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | > 90% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ] |
Official Symbol | IL18 |
Synonyms | IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320; |
Gene ID | 3606 |
mRNA Refseq | NM_001243211 |
Protein Refseq | NP_001230140 |
MIM | 600953 |
UniProt ID | Q14116 |
◆ Recombinant Proteins | ||
IL18-5756H | Recombinant Human IL18 protein, His-tagged | +Inquiry |
IL18-1011H | Recombinant Horse IL18 Protein, His-tagged | +Inquiry |
IL18-186H | Recombinant Human IL18 protein | +Inquiry |
IL18-334R | Recombinant Rhesus IL18 protein, His-tagged | +Inquiry |
IL18-2943H | Recombinant Human IL18 Protein (Tyr37-Asp193), C-His tagged | +Inquiry |
◆ Native Proteins | ||
IL18-01D | Recombinant Dog IL18 Protein, Tag Free | +Inquiry |
◆ Cell & Tissue Lysates | ||
IL18-344HCL | Recombinant Human IL18 lysate | +Inquiry |
Not For Human Consumption!
Inquiry
- Reviews (0)
- Q&As (1)
Ask a Question for All IL18 Products
Required fields are marked with *
My Review for All IL18 Products
Required fields are marked with *