Recombinant Human IL1A protein, GST-tagged

• Cat.No.: IL1A-14167H
• Product Overview: Recombinant Human IL1A protein (113-271 aa) fused to GST-tag and was expressed in E.coli.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
• Source: E.coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.). 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Molecular Mass: 44 kDa
• Protein length: 159
• AA Sequence: SAPFSFLSNVKYNFMRIIKYEFILN DALNQSIIRANDQYLTAAALHNLDE AVKFDMGAYKSSKDDAKITVILRIS KTQLYVTAQDEDQPVLLKEMPEIPK TITGSETNLLFFWETHGTKNYFTSV AHPNLFIATKQDYWVCLAGGPPSIT DFQILENQA
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Samples are stable for up to twelve months from date of receipt at -20 centigrade to -80 centigrade
  Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Gene Information

• Gene Name: IL1A
• Official Symbol: IL1A
• Synonyms: IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha
• Gene ID: 3552
• mRNA Refseq: NM_000575.5
• Protein Refseq: NP_000566.3
• MIM: 147760
• UniProt ID: P01583