Recombinant Human IL1F5 293 Cell Lysate
Cat.No. : | IL36RN-5239HCL |
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Description : | Antigen standard for interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Tag : | Non |
Gene Name : | IL36RN interleukin 36 receptor antagonist [ Homo sapiens ] |
Official Symbol : | IL36RN |
Synonyms : | FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1; IL1RP3; IL36RA; FIL1(DELTA); interleukin-36 receptor antagonist protein; IL-1ra homolog 1; interleukin-1 HY1; IL-1 related protein 3; interleukin-1-like protein 1; IL1F5 (Canonical product IL-1F5a); interleukin 1 family, member 5 (delta); interleukin-1 receptor antagonist homolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta) |
Gene ID : | 26525 |
mRNA Refseq : | NM_173170 |
Protein Refseq : | NP_775262 |
MIM : | 605507 |
UniProt ID : | Q9UBH0 |
Chromosome Location : | 2q14 |
Function : | cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding |
Products Types
◆ Lysates | ||
IL36RN-5240HCL | Recombinant Human IL1F5 293 Cell Lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewWhen conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.
IL36RN has high specific activity and can exert strong biological effect at low concentration.
The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.
Q&As (6)
Ask a questionThe mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.
The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.
Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.
This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.
Can be used in clinical trials and therapy.
IL36RN has sustained and stable biological activity.
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