Recombinant Human IL1F5 293 Cell Lysate

Cat.No. : IL36RN-5239HCL
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Description : Antigen standard for interleukin 1 family, member 5 (delta) (IL1F5), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : IL36RN interleukin 36 receptor antagonist [ Homo sapiens ]
Official Symbol : IL36RN
Synonyms : FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1; IL1RP3; IL36RA; FIL1(DELTA); interleukin-36 receptor antagonist protein; IL-1ra homolog 1; interleukin-1 HY1; IL-1 related protein 3; interleukin-1-like protein 1; IL1F5 (Canonical product IL-1F5a); interleukin 1 family, member 5 (delta); interleukin-1 receptor antagonist homolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
Gene ID : 26525
mRNA Refseq : NM_173170
Protein Refseq : NP_775262
MIM : 605507
UniProt ID : Q9UBH0
Chromosome Location : 2q14
Function : cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding

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Reviews
05/31/2021

    When conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.

    01/29/2019

      IL36RN has high specific activity and can exert strong biological effect at low concentration.

      01/11/2019

        The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.

        Q&As (6)

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        What is the action mechanism of IL36RN? 06/11/2022

        The mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.

        How to ensure the purity of IL36RN when preparing it? 11/22/2021

        The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.

        IL36RN production process is what? 03/24/2020

        Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.

        What is the difference between IL36RN protein and IL36α, β, γ? 03/22/2020

        This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.

        Can recombinant IL36RN be used in clinical trials and therapeutics? 09/24/2019

        Can be used in clinical trials and therapy.

        Does IL36RN have sustained and stable biological activity? 03/24/2019

        IL36RN has sustained and stable biological activity.

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