Recombinant Human IL36RN protein
Cat.No. : | IL36RN-512H |
Product Overview : | Recombinant Human IL36RN protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
- Download
Description : | Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91 % a.a. sequence identity with murine IL-36RA. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 20 ng/ml of recombinant human IL-36 beta. |
Molecular Mass : | Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids. |
Protein length : | 154 |
AA Sequence : | VLSGALCFRMKDSALKVLYLHNNQL LAGGLHAGKVIKGEEISVVPNRWLD ASLSPVILGVQGGSQCLSCGVGQEP TLTLEPVNIMELYLGAKESKSFTFY RRDMGLTSSFESAAYPGWFLCTVPE ADQPVRLTQLPENGGWNAPITDFYF QQCD |
Endotoxin : | Less than 1 EU/µg of rHuIL-36RA as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Tag : | Non |
Gene Name : | IL36RN |
Official Symbol : | IL36RN |
Synonyms : | IL36RN; interleukin 36 receptor antagonist; IL1F5, interleukin 1 family, member 5 (delta); interleukin-36 receptor antagonist protein; family of interleukin 1 delta; FIL1; FIL1(DELTA); FIL1D; IL 1 related protein 3; IL 1F5; IL1HY1; IL1L1; IL1RP3; IL36RA; interleukin 1 HY1; interleukin 1 receptor antagonist homolog 1; MGC29840; IL-1ra homolog 1; interleukin-1 HY1; IL-1 related protein 3; interleukin-1-like protein 1; family of interleukin 1-delta; IL1F5 (Canonical product IL-1F5a); interleukin 1 family, member 5 (delta); interleukin-1 receptor antagonist homolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta); IL1F5; PSORP; |
Gene ID : | 26525 |
mRNA Refseq : | NM_012275 |
Protein Refseq : | NP_036407 |
MIM : | 605507 |
UniProt ID : | Q9UBH0 |
Products Types
◆ Recombinant Protein | ||
IL36RN-118H | Active Recombinant Human IL36RN Protein (Met1-Asp155), C-His tagged, Animal-free, Carrier-free | +Inquiry |
IL36RN-200H | Recombinant Active Human IL36RN Protein, His-tagged(C-ter) | +Inquiry |
IL36RN-2009H | Recombinant Human IL36RN Protein, His-tagged | +Inquiry |
Il36rn-3505M | Recombinant Mouse Il36rn Protein, Myc/DDK-tagged | +Inquiry |
IL36RN-5185H | Recombinant Human IL36RN Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
IL36RN-5240HCL | Recombinant Human IL1F5 293 Cell Lysate | +Inquiry |
IL36RN-5239HCL | Recombinant Human IL1F5 293 Cell Lysate | +Inquiry |
Related Gene
Not For Human Consumption!
Inquiry
- Reviews
- Q&As
Customer Reviews (3)
Write a reviewWhen conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.
IL36RN has high specific activity and can exert strong biological effect at low concentration.
The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.
Q&As (6)
Ask a questionThe mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.
The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.
Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.
This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.
Can be used in clinical trials and therapy.
IL36RN has sustained and stable biological activity.
Ask a Question for All IL36RN Products
Required fields are marked with *
My Review for All IL36RN Products
Required fields are marked with *
Inquiry Basket