Active Recombinant Human IL1RAP protein, hFc-tagged, Low Endotoxin

• Cat.No.: IL1RAP-627H
• Product Overview: Recombinant Human IL1RAP protein(Q9NPH3-1)(Ser21-Glu359), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 21-359 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-1R3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 18.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 65.8 kDa. Due to glycosylation, the protein migrates to 80-95 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL1RAP interleukin 1 receptor accessory protein [ Homo sapiens ]
• Official Symbol: IL1RAP
• Synonyms: IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein; C3orf13; IL 1RAcP; IL1R3; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta; IL-1RAcP; FLJ37788
• Gene ID: 3556
• mRNA Refseq: NM_001167928
• Protein Refseq: NP_001161400
• MIM: 602626
• UniProt ID: Q9NPH3