Recombinant Mouse IL1RAP Protein, His-tagged

• Cat.No.: Il1rap-443M
• Product Overview: Recombinant Mouse Il1rap (NP_598864.1) (Met1-Lys350) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Description: Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a a cytokine receptor which binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by ass centigradeiating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.
• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser 21
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse Il1rap consists of 341 amino acids and predicts a molecular mass of 39.7 kDa.
• Protein length: Met1-Lys350
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Il1rap interleukin 1 receptor accessory protein [ Mus musculus ]
• Official Symbol: Il1rap
• Synonyms: IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein; IL-1 receptor accessory protein; AI255955; AV239853; IL-1RAcP; 6430709H04Rik
• Gene ID: 16180
• mRNA Refseq: NM_001159317
• Protein Refseq: NP_001152789

Reviews

05/25/2021

High solubility.

07/22/2019

Good for intracellular staining.

05/13/2018

Effective in apoptosis assays.

Q&As

How can single-cell RNA sequencing and proteomics uncover IL1RAP's interactions with immune receptors within complex networks?

Single-cell RNA sequencing and proteomics unveil IL1RAP's interactions in networks.

Can genome-wide mapping and functional assays reveal the epigenetic regulation and context-specific expression of the IL1RAP gene?

Genome mapping and assays unveil IL1RAP's epigenetic control.

How can we unravel IL1RAP's varied distribution across cell types and tissues, clarifying its functional versatility?

Innovative methods clarify IL1RAP's distribution diversity, revealing its functional adaptability.

How do advanced imaging and genetic knockout models shed light on IL1RAP's role in immune cell dynamics and functions?

Advanced imaging and genetic models illuminate IL1RAP's immune role.

What insights do computational simulations and in vitro experiments provide into IL1RAP's interaction kinetics and stability?

Simulations and experiments reveal IL1RAP's interaction details.

Do engineered cellular models clarify IL1RAP's mechanisms in inflammasome activation and cytokine release?

Engineered cell models shed light on IL1RAP's role in inflammation.

Can structural biology techniques elucidate IL1RAP's conformational changes and ligand interactions in inflammation?

Structural techniques provide insights into IL1RAP's ligand interactions.

What strategies unveil the influence of post-translational modifications on IL1RAP's localization and signaling pathways?

Strategies uncover post-translational effects on IL1RAP's signaling.

How do organ-on-chip technologies mimic tissue microenvironments to explore IL1RAP's impact on signaling pathways?

Organ-on-chip tech explores IL1RAP's impact on pathways.

What innovative methods decode IL1RAP isoforms in different cells, revealing their impact on cellular responses?

Novel techniques decode IL1RAP isoforms, exposing their influence on cell responses.