Recombinant Human IL1RL1 cell lysate
Cat.No. : | IL1RL1-1883HCL |
Product Overview : | Human IL1RL1 / ST2 ( isoform A ) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | IL1RL1 interleukin 1 receptor-like 1 [ Homo sapiens ] |
Official Symbol : | IL1RL1 |
Synonyms : | IL1RL1; interleukin 1 receptor-like 1; interleukin-1 receptor-like 1; DER4; FIT 1; homolog of mouse growth stimulation expressed; IL33R; ST2; ST2L; ST2V; T1; growth stimulation-expressed; interleukin 1 receptor-related protein; homolog of mouse growth stimulation-expressed; FIT-1; MGC32623; |
Gene ID : | 9173 |
mRNA Refseq : | NM_003856 |
Protein Refseq : | NP_003847 |
MIM : | 601203 |
UniProt ID : | Q01638 |
Chromosome Location : | 2q12 |
Function : | cytokine receptor activity; interleukin-1 receptor activity; interleukin-33 binding; protein binding; receptor activity; receptor signaling protein activity; |
Products Types
◆ Recombinant Protein | ||
IL1RL1-536H | Recombinant Human IL1RL1 protein, His-tagged | +Inquiry |
IL1RL1-2690R | Recombinant Rat IL1RL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
IL1RL1-317H | Recombinant Human IL1RL1 Protein, His-tagged | +Inquiry |
IL1RL1-195H | Recombinant Human IL1RL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
IL1RL1-1009H | Active Recombinant Human ST2 Protein | +Inquiry |
◆ Lysates | ||
IL1RL1-2481HCL | Recombinant Human IL1RL1 cell lysate | +Inquiry |
IL1RL1-001MCL | Recombinant Mouse IL1RL1 cell lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewHigh specificity in ELISA.
Suitable for flow cytometry.
Effective in allergy models.
Q&As (10)
Ask a questionIL1RL1 has multiple splice variants that differ in signaling capabilities.
IL1RL1 suppresses allergic responses by inhibiting mast cell activation.
IL1RL1 is implicated in rheumatoid arthritis.
IL1RL1 increases the production of IL-4 and IL-13.
IL1RL1 is primarily membrane-bound.
IL1RL1 promotes Th2 cell differentiation.
IL1RL1 binds to IL-33 and IL-36 to initiate signaling.
IL1RL1 expression is altered in lung and breast cancer.
IL1RL1 undergoes glycosylation and phosphorylation.
IL1RL1 expression is upregulated in asthmatic conditions.
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