Active Recombinant Human IL27 protein, His-tagged

• Cat.No.: IL27-311H
• Product Overview: Recombinant Human IL27 complex composed of IL27B subunit (NP_005746.2) (Arg 21 - Lys 229) and the N-terminally truncated IL27p28 (IL27A) subunit (NP_663634.2) (Phe 29-Pro 243) linked by an polypeptide linker, fused with a polyhistidine tag at the C-terminus,

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 29-243 aa&21-229 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4).
• Bio-activity: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 22.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL27 interleukin 27 [ Homo sapiens ]
• Official Symbol: IL27
• Synonyms: IL27; interleukin 27
• Gene ID: 144841