| Cat.No. : |
IL2RA-170H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human IL2RA (Glu22-Cys213) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293). |
| Description : |
Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
human IgG1 Fc |
| Predicted N Terminal : |
Glu22 |
| Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 48.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
Glu22-Cys213 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of IL2RA-170H : |
Human IL-2 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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| Bioactivity-ELISA of IL2RA-170H : |
Immobilized Human IL-2 R alpha, Fc Tag at 2μg/mL (100 μL/well) can bind Human IL-2, Tag Free with a linear range of 0.2-1.56 ng/mL (QC tested).
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| Bioactivity-BLI : |
Loaded Human IL-2 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 43.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.