Active Recombinant Human IL3 Protein, Pre-aliquoted

• Cat.No.: IL3-07H
• Product Overview: Recombinant Human IL3 Protein, Ala20 - Phe152, with and without an N-terminal Met, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL.
• Molecular Mass: Predicted Molecular Mass:15 kDa
  SDS-PAGE: 14 kDa, reducing conditions
• Protein length: Ala20-Phe152, with and without an N-terminal Met
• N-terminal Sequence Analysis: Ala20 and Met
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: IL3 interleukin 3 [ Homo sapiens (human) ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3; IL-3; MCGF; MULTI-CSF; interleukin-3; P-cell stimulating factor; colony-stimulating factor, multiple; hematopoietic growth factor; mast-cell growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700