Recombinant Human IL31 protein, His-tagged

• Cat.No.: IL31-311H
• Product Overview: Recombinant Human IL31 protein(NP_001014358.1) (Met1-Thr164) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr164
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to induce STAT3 activation in U‑87 cells. The ED50 for this effect is 11-45 ng/mL.
• Molecular Mass: The recombinant human IL31 consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 31.8 KDa band in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL31 interleukin 31 [ Homo sapiens ]
• Official Symbol: IL31
• Synonyms: IL31; interleukin 31; interleukin-31; IL 31; IL-31
• Gene ID: 386653
• mRNA Refseq: NM_001014336
• Protein Refseq: NP_001014358
• MIM: 609509
• UniProt ID: Q6EBC2