Recombinant Human IL3RA Protein, His-Avi-tagged, Biotinylated

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Recombinant Human IL3RA Protein, His-Avi-tagged, Biotinylated

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Cat.No. : IL3RA-156H
Product Overview : Biotinylated Human IL-3 R alpha, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305 (Accession # P26951-1).
Description : Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.
Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Thr 19
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Molecular Mass : The protein has a calculated MW of 36.7 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Thr 19 - Arg 305
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE :

SEC-MALS :

Bioactivity-ELISA :

Immobilized Biotinylated Human IL-3 R alpha, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL.

Gene Name : IL3RA interleukin 3 receptor, alpha (low affinity) [ Homo sapiens ]
Official Symbol : IL3RA
Synonyms : IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; CD123 antigen; CD123; hIL3Ra; hIL-3Ra; MGC34174; IL-3 R alpha
Gene ID : 3563
mRNA Refseq : NM_002183
Protein Refseq : NP_002174
MIM : 308385
UniProt ID : P26951

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