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Recombinant Human IL3RA Protein, His-Avi-tagged, Biotinylated
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| Cat.No. : | IL3RA-156H |
| Product Overview : | Biotinylated Human IL-3 R alpha, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305 (Accession # P26951-1). |
| Description : | Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | His-Avi |
| Predicted N Terminal : | Thr 19 |
| Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Molecular Mass : | The protein has a calculated MW of 36.7 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE). |
| Protein length : | Thr 19 - Arg 305 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >95% as determined by SDS-PAGE. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE : |
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| SEC-MALS : |
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| Bioactivity-ELISA : |
Immobilized Biotinylated Human IL-3 R alpha, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL. |
| Gene Name : | IL3RA interleukin 3 receptor, alpha (low affinity) [ Homo sapiens ] |
| Official Symbol : | IL3RA |
| Synonyms : | IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; CD123 antigen; CD123; hIL3Ra; hIL-3Ra; MGC34174; IL-3 R alpha |
| Gene ID : | 3563 |
| mRNA Refseq : | NM_002183 |
| Protein Refseq : | NP_002174 |
| MIM : | 308385 |
| UniProt ID : | P26951 |
◆ Recombinant Protein
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| Il3ra-918MP | Recombinant Mouse Il3ra protein, Fc-tagged, R-PE labeled | +Inquiry |
| IL3RA-1120H | Recombinant Human IL3RA Protein (Met1-Arg305), HlgG1 Fc-tagged | +Inquiry |
| IL3RA-121HP | Recombinant Human IL3RA protein, Fc-tagged, R-PE labeled | +Inquiry |
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◆ Lysates
| IL3RA-742CCL | Recombinant Canine IL3RA cell lysate | +Inquiry |
| IL3RA-2433HCL | Recombinant Human IL3RA cell lysate | +Inquiry |
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