| Cat.No. : |
IL3RA-156H
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Biotinylated Human IL-3 R alpha, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305 (Accession # P26951-1). |
| Description : |
Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His-Avi |
| Predicted N Terminal : |
Thr 19 |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Molecular Mass : |
The protein has a calculated MW of 36.7 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE). |
| Protein length : |
Thr 19 - Arg 305 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE : |
|
| SEC-MALS : |
|
| Bioactivity-ELISA : |
Immobilized Biotinylated Human IL-3 R alpha, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL.
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
