| Description : |
Interleukin-6 receptor subunit alpha (IL-6Ra), also known as membrane glycoprotein 80 (gp80), IL-6R 1 or CD126, is a single-pass type I membrane protein of the interleukin receptor family. Human IL-6Ra is an 80 kDa receptor component that binds IL-6 with low affinity and forms one-half of the IL-6R receptor complex. IL-6Ra associates with a non-ligand binding 130 kDa glycoprotein signal-transducing component (CD130; gp130) for high-affinity binding to IL-6 (1-4). When bound to IL-6, the IL-6R complex forms a trimer, which homodimerizes to form a hexameric cellular signaling complex (1, 4). Human IL-6Ra consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain. Within the mature ECD, human IL-6Ra shares 51% and 52% amino acid sequence identity with mouse and rat IL-6Ra, respectively. Soluble forms of IL-6Ra can be generated via ADAM10 and ADAM17 cleavage of membrane bound IL-6Ra, alternative mRNA splicing and microvesicle release (3, 4). Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn's disease (6, 7). Our Avi-tag Biotinylated IL-6Ra features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity. |
| Source : |
CHO cells |
| Species : |
Human |
| Tag : |
His/Avi |
| Predicted N Terminal : |
Leu20 |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Human IL6 is immobilized at 0.25 µg/mL (100 µL/ well), Biotinylated Recombinant Human IL6R His-tag Avi‑tag binds with an ED50 of 0.1-1 μg/mL. |
| Molecular Mass : |
65-80 kDa, under reducing conditions |
| Protein length : |
Leu20-Pro365 |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 1 mg/mL in PBS. |
