Recombinant Human IL7R protein, Fc-His-tagged

• Cat.No.: IL7R-788H
• Product Overview: Recombinant Human IL7R protein(NP_002176.2)(Met 1-Gly 236) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-236 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its ability to bind biotinylated mouse TSLP-his in functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL7 at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch, The EC50 of human IL7Ra-Fch is 15.2-35.6 ng/ml.
• Molecular Mass: The recombinant human IL7Rα/Fc is a disulfide-linked homodimer. The reduced monomer consists of 464 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL7Rα/Fc monomer is apximately 65-75 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_002176.2
• Unit ID: P16871
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P16871

Gene Information

• Gene Name: IL7R interleukin 7 receptor [ Homo sapiens ]
• Official Symbol: IL7R
• Synonyms: IL7R; interleukin 7 receptor; interleukin-7 receptor subunit alpha; CD127; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; ILRA; IL7RA; CDW127; IL-7R-alpha
• Gene ID: 3575
• mRNA Refseq: NM_002185
• protein Refseq: NP_002176
• MIM: 146661