Recombinant Human ILK protein, His-tagged

• Cat.No.: ILK-3586H
• Product Overview: Recombinant Human ILK protein(102-451 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 102-451 aa
• AA Sequence: VPLHYACFWGQDQVAEDLVANGALV SICNKYGEMPVDKAKAPLRELLRER AEKMGQNLNRIPYKDTFWKGTTRTR PRNGTLNKHSGIDFKQLNFLTKLNE NHSGELWKGRWQGNDIVVKVLKVRD WSTRKSRDFNEECPRLRIFSHPNVL PVLGACQSPPAPHPTLITHWMPYGS LYNVLHEGTNFVVDQSQAVKFALDM ARGMAFLHTLEPLIPRHALNSRSVM IDEDMTARISMADVKFSFQCPGRMY APAWVAPEALQKKPEDTNRRSADMW SFAVLLWELVTREVPFADLSNMEIG MKVALEGLRPTIPPGISPHVCKLMK ICMNEDPAKRPKFDMIVPILEKMQD
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: ILK integrin-linked kinase [ Homo sapiens ]
• Official Symbol: ILK
• Synonyms: ILK; integrin-linked kinase; integrin-linked protein kinase; ILK-1; p59ILK; integrin-linked kinase-2; 59 kDa serine/threonine-protein kinase; P59; ILK-2; DKFZp686F1765
• Gene ID: 3611
• mRNA Refseq: NM_001014794
• Protein Refseq: NP_001014794
• MIM: 602366
• UniProt ID: Q13418