Recombinant Human Interferon, Gamma-Inducible Protein 30, His-tagged

• Cat.No.: IFI30-7185H
• Product Overview: Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW: 22.5 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 58-232aa
• Description: IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT.
• Molecular Mass: 22.5 kDa (199aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK PLEDQTQLLT LVCQLYQGK
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: IFI30 interferon, gamma-inducible protein 30 [ Homo sapiens ]
• Official Symbol: IFI30
• Synonyms: IFI30; interferon, gamma-inducible protein 30; GILT; IP30; IFI-30; gamma-interferon-inducible lysosomal thiol reductase; legumaturain; gamma-interferon-inducible protein IP-30; interferon gamma-inducible protein 30 preproprotein; MGC32056; EC 1.8; Legumaturain
• Gene ID: 10437
• mRNA Refseq: NM_006332
• Protein Refseq: NP_006323
• MIM: 604664
• UniProt ID: P13284
• Chromosome Location: 19p13.1
• Pathway: Adaptive Immune System; Antigen processing and presentation; Cytokine Signaling in Immune system; Immune System; Interferon Signali
• Function: oxidoreductase activity, acting on a sulfur group of donors